Dermatology and Melanoma

Biography

Abstract

The present work was focused with objective to optimize the ellagic acid loaded phytocomplex using complexation method. For optimization process, Box–Behnken design, with a 33 design and a total of 17 experimental runs, performed in combination with response surface methodology (RSM) were applied and effect of process variables on ellagic acid loaded phytocomplex were observed. Influence of three independent variables in the preparation of phytocomplex was investigated to get the best formulation. Independent variables covered concentration variable of lipid concentration, drug concentration and rotation time. Dependent variables included like percentage yield (PY%), drug release (%DR), and entrapment efficiency (%EE) of the prepared phytocomplex. Dialysis method was used to separate the drug from uncomplexation. Phytocomplex was optimized using response surface methodology. Accordingly, concentration of drug (100 mg), phospholipid (75 mg) and rotation time (40 minutes) were optimized and finalized as the best for optimized ellagic acid-loaded phytocomplex. In vitro characterization of the ellagic acid-loaded phytocomplex was done through some parameters including the PY%, DR% and EE%; the resulting values of 82.10%, 78.35%, and 78.36% were found to be standard characterized values respectively. It is concluded that ellagic acid-loaded phytocomplex play significant role to achieve sustained release of drug in wound areas.